Mechanical Characterization Research Articles

Mechanistic Characterization of Cancer Associated Fibroblast Depletion via an Antibody-Drug Conjugate Targeting Fibroblast Activation Protein.

Cancer-associated fibroblasts (CAFs) are a prominent cell type within the tumor microenvironment where they are known to promote cancer cell growth and survival, angiogenesis, drug resistance, and immunosuppression. The transmembrane prolyl protease Fibroblast Activation Protein (FAP) is expressed on the surface of highly pro-tumorigenic CAFs found in the stroma of nearly every cancer of epithelial origin. The widespread expression of FAP has made it an attractive therapeutic target based on the underlying hypothesis that eliminating pro-tumorigenic CAFs will disrupt the crosstalk between components of TME resulting in cancer cell death and immune infiltration. This hypothesis, however, has never been directly proven. To eliminate FAP-expressing CAFs, we developed an antibody-drug conjugate (ADC) using our anti-FAP antibody, huB12, coupled to a monomethyl auristatin E (huB12-MMAE) payload. After determining that huB12 was an effective targeting vector, we found that huB12-MMAE potently eliminated FAP-expressing cells as monocultures in vitro and significantly prolonged survival in vivo using a xenograft engineered to overexpress FAP. We investigated the effects of selectively eliminating CAFs using a layered, open microfluidic cell co-culture platform, known as the Stacks. Analysis of mRNA and protein expression found that treatment with huB12-MMAE resulted in the increased secretion of the pro-inflammatory cytokines IL6 and IL8 by CAFs and an associated increase in expression of pro-inflammatory genes in cancer cells. We also detected increased secretion of CSF1, a cytokine involved in myeloid recruitment and differentiation. Our findings suggest that the mechanism of FAP-targeted therapies are through effects on the immune microenvironment and anti-tumor immune response.

Lipid-derived electrophiles inhibit the function of membrane channels during ferroptosis

The therapeutic targeting of ferroptosis requires full understanding of the molecular mechanism of this regulated cell death pathway. While lipid-derived electrophiles (LDEs), including 4-hydroxy-2-nonenal (4-HNE), are important biomarkers of ferroptosis, a functional role for these highly reactive species in ferroptotic cell death execution has not been established. Here, through mechanistic characterization of LDE-detoxification impairment, we demonstrate that LDEs mediate altered protein function during ferroptosis. Applying live cell fluorescence imaging, we first identified that export of glutathione-LDE-adducts through multidrug resistance-associated protein (MRP) channels is inhibited following exposure to a panel of ferroptosis inducers (FINs) with different modes of action (type I-IV FINs erastin, RSL3, FIN56, and FINO2). This channel inhibition was recreated by both initiation of lipid peroxidation and treatment with 4-HNE. Importantly, treatment with radical-trapping antioxidants prevented impaired LDE-adduct export when working with both FINs and lipid peroxidation initiators but not 4-HNE, pinpointing LDEs as the cause of this inhibited MRP activity observed during ferroptosis. Our findings, when combined with reports of widespread LDE alkylation of key proteins following ferroptosis induction, including MRP1, set a precedent for LDEs as critical mediators of ferroptotic cell damage. Lipid hydroperoxide breakdown to form truncated phospholipids and LDEs may fully explain membrane permeabilization and modified protein function downstream of lipid peroxidation, offering a unified explanation of the molecular cell death mechanism of ferroptosis.

Mechanical characterization of 3D-Printed carbon fiber-reinforced polymer composites and pure polymers: Tensile and compressive behavior analysis

Mechanical characterization of 3D-Printed carbon fiber-reinforced polymer composites and pure polymers: Tensile and compressive behavior analysis

Electrochemical characterization of localized corrosion mechanism of ALD Al2O3-coated copper for microelectronic application

To achieve performance and reliability in microelectronics devices, copper interconnections must be protected from aggressive environment during the manufacturing process and throughout the life of the devices. Atomic Layer Deposition Al2O3 thin films has been used as a compatible layer with key integration process steps, as well as being corrosion-resistant in neutral media. However, electrochemical tests in aggressive environment have revealed that the alumina prematurely failed to protect copper from localized corrosion. In this study, local and global electrochemical techniques have been employed to evaluate the reactivity of copper at multiple scales and to elucidate the underlying corrosion mechanisms.

Mechanical and thermal characterization of elastomer modified polypropylene hybrid composites reinforced with hazelnut shell and wollastonite fillers

AbstractThe demand for sustainable and recyclable materials in industrial applications has led to a surge in interest in green composites, particularly those incorporating natural fillers derived from agro‐food industry waste. This study investigates the mechanical and thermal properties of polypropylene (PP) hybrid composites filled with hazelnut shell and wollastonite, along with the effect of styrene‐ethylene/butylene‐styrene (SEBS) and SEBS‐g‐maleic anhydride (SEBS‐g‐MA) compatibilizers. Various characterization techniques, including tensile, flexural, and impact tests, as well as differential scanning calorimetry (DSC), thermogravimetry (TGA), and scanning electron microscopy (SEM) analyses, were employed to evaluate the composites. Results demonstrate that the addition of wollastonite significantly improves mechanical properties, while hazelnut shell filler affects thermal behavior and stability. SEBS and SEBS‐g‐MA compatibilizers enhance impact resistance; however, they lead to a decrease in other mechanical properties. DSC and TGA analyses demonstrate changes in crystallization behavior and thermal stability due to filler and compatibilizer incorporation. SEM microstructure images show the distribution of SEBS and SEBS‐g‐MA within the composite structure, affecting mechanical and thermal properties. Overall, this study highlights the importance of filler selection, compatibilizer addition, and their distribution in attaining desired properties for industrial applications. Future research should focus on optimizing formulations for specific uses and assessing long‐term performance under real‐world conditions.

An integrated experimental and analytical approach on mechanical characterization of advanced powder metallurgy aluminium metal matrix composites reinforced with different particulates

Abstract Over the last few decades, “Discontinuously Reinforced Particulate Composites (DRPCs)” are a popular class of composite materials with considerable challenge in processing, characterization and machinability because of their increased strength-weight ratio, stiffness, specific strength and oxidization when compared to various metals and their alloys. This paper discusses experimental and numerical investigation on mechanical characteristics of aluminum metal matrix reinforced with various reinforcement particulates such as silicon carbide, aluminium oxide, and zirconium oxide, compaction pressure (kN) and hold time (s) based on Design of Experiments (DOE) and Finite Element Analysis. Initially this paper discusses the process optimization of Aluminum Matrix reinforced with different particulates experimentally to identify the favourable processing conditions by varying reinforcement materials, compaction pressure (kN) and hold time (s) based on TDOE (Taguchi’s Design of Experiments). Further, this paper concentrates to determine “maximum principal stress, equivalent elastic strain and equivalent (von-mises) stress” based on Finite Element Analysis (ANSYS Workbench-2023R1). The results of the experimentation showed that the highest hardness values were achieved with ZrO2 reinforcement material. Increasing the compaction pressure from 8 to 12 kN resulted in a slight decrease in surface roughness and porosity. Higher compaction pressures have assumed to facilitate better particle distribution and improved interfacial bonding, leading to smoother surfaces and lower void content. The simulation results showed that the maximum principal stress achieved were (2235.8 Mpa) SiC, (3444.4 Mpa) Al2O3, and (3582.5 MPa) ZrO2. The equivalent elastic strain achieved was (0.2488) SiC, (0.2421) Al2O3 and (0.262) ZrO2. The equivalent (Von Mises) stress achieved was (28751 MPa) for SiC, (24880 MPa) for ZrO2 and (26972 Mpa) for Al2O3. This experimentation and simulation demonstrated that the PM process can be used to fabricate DRAMMC with different reinforcement particulates.

Mechanical Characterization of Hybrid Bagasse/Eggshell/E-glass Fiber-based Polyester Composite

Currently, numerous researchers, scientists, and industrialists are drawn towards utilizing natural fillers rather than synthetic ones because of their environmentally-friendly characteristics, lower density per unit volume and favorable mechanical attributes. Several studies have demonstrated that using organic filler in polyester matrix composites, instead of disposing of them in landfills, has various advantages. The current study focuses on the investigation of the mechanical characteristics of composite material made from a combination of bagasse, eggshell, and e-glass fibers exhibit characteristics such as strength under tension, strength under bending, resistance to indentation, and mass per unit volume. Different weight percentages of eggshell/bagasse were used to make the material using the hand lay-up technique (3wt%, 6wt%, 9wt%, and 12wt%), with 19wt% of glass fiber mixed in the resin. The use of inorganic fillers like calcium carbonate, a non-renewable mineral that may be expensive and environmentally hazardous to extract and process, is being reduced in favor of organic fillers like eggshell and bagasse, which are inexpensive and readily available. Specimens were generated by interposing fiber waste amidst chopped strand glass fiber mats, with variations in the bagasse, eggshell and polyester content, while keeping the amount of E-glass fiber constant. Afterward, the specimens were trimmed to the appropriate dimensions and subjected to analysis with a universal apparatus. The research showed that enhancing the quantity of bagasse/eggshell fiber within the hybrid composite resulted in an enhancement of its mechanical characteristics. The best reinforcement effect was observed in a hybrid composite with 6% bagasse/eggshell fiber.

Investigation on mechanical and thermal behaviour of graphene bonded carbon/Kevlar hybrid fabric polymer composites

The present research is aimed to understand the influence of graphene-reinforced carbon/Kevlar hybrid fabric epoxy composites. Samples HG0, HG2, HG4, and HG6 are fabricated using four layers of fabric mat and with varying graphene weight percentage as 0, 2, 4, and 6 wt.%, respectively. Mechanical characterizations such as hardness, tensile, and flexural strength were individually assessed. Thermal stability is evaluated through thermos-gravimetric analysis. Composite HG6 with high filler loading showed 71% and 114.4% greater tensile and flexural strength compared to unfilled HG0 specimen. Similarly, HG6 exhibited greater thermal stability. Inclusion of graphene particles in higher concentration improved the interfacial bonding between fibre/matrix/filler system. Fracture surfaces are analysed using scanning electron microscopy to understand the failure mechanisms.

Micro and macro mechanical characterization of artificial cemented granular materials

Micro and macro mechanical characterization of artificial cemented granular materials

Solder joint reliability performance study and shear characterization of low-Ag SAC lead-free solders for handheld application

As handheld electronic products move toward smaller and thinner form factor, the demand of high reliability under stringent field usage also increasing. Drop test, based on the JEDEC JESD22-B111A, accompanied with temperature cycling (TC) test, based on JEDEC JESD22-A104F, are two of the most critical board-level reliability tests for handheld makers. In terms of solder joint reliability (SJR), total 7 candidates of LF35-liked lead-free solder alloys are investigated for its drop performance, and TC reliability evaluation. Among 7 solders, leg 4 and 5 exhibit both decent drop and TC behavior, which are likely owing to the silver (Ag) precipitation hardening and bismuth (Bi) solid solution hardening effect respectively. In addition, the shearing mechanical characterization of 7 solder alloys are being studied. Shear strength of leg 4 and 5 shows higher strength value than others. In terms of failure analysis under drop test, three main fracture modes are observed, including via cracking at PCB side, solder/intermetallic compound (IMC) cracking at package side, and Cu trace broken at package side. Furthermore, in terms of failure modes of TC test, solder/IMC cracking at package side are the main fracture modes. In summary, this study provides an overall reliability performance mapping for 7 solders, and list out a table comprising of shear strength, void% analysis, TC reliability, and drop reliability performance for each LF35 solders which lays out potential future solder alloy to meet higher handheld phone drop reliability margin.

Electrical and mechanical limits of ex situ MgB2 wires for cabling

One of the objectives of the SCARLET project is to develop and industrially manufacture superconducting MgB2 cables cooled by liquid hydrogen. The ex situ powder-in-tube MgB2 wires manufactured by ASG are considered for the cable design that can carry DC current of 20 kA. These braided superconducting wires, containing brittle filaments, require high current. Thus, the study of the electro-mechanical properties of MgB2 wires is crucial for the cable design and its functional use. Superconducting wires have to withstand all the stresses applied during the cabling process, installation, and operations at the temperature of around 20 K. Hence, several configurations of MgB2/Ni/Monel composite wires have been subjected to detailed electrical and mechanical characterizations, which allow the estimation of the stress limits during the manufacturing of the designed cable. These experiments demonstrated that the maximal tensile stress applied to the wire at room temperature should be below 180–200 MPa, and safety bending observed for the outer filament strains was below 0.3%–0.35%. It is also revealed that the limit of acceptable torsion (expressed by the twist pitch to wire diameter L t/d w) is affected by the filament architecture and wire diameter. This limit should be above 100 for 1 mm wire and above 150 for 1.53 mm wire.

The Metabolism and Disposition of Brepocitinib in Humans and Characterization of the Formation Mechanism of an Aminopyridine Metabolite.

<s><s></s></s> Brepocitinib is an oral once-daily Janus kinase 1 and Tyrosine kinase 2 selective inhibitor currently in development for the treatment of several autoimmune disorders. Mass balance and metabolic profiles were determined using accelerator mass spectrometry in six healthy male participants following a single oral 60 mg dose of 14C-brepocitinib (~300 nCi). The average mass balance recovery was 96.7% {plus minus} 6.3% with the majority of dose (88.0% {plus minus} 8.0%) recovered in urine and 8.7% {plus minus} 2.1% of the dose in recovered in feces. Absorption of brepocitinib was rapid, with maximal plasma concentrations of total radioactivity and brepocitinib achieved within 0.5 hours after dosing. Circulating radioactivity consisted primarily of brepocitinib (47.8%) and metabolite M1 (37.1%) derived from hydroxylation at the C5' position of the pyrazole ring. Fractional contributions to metabolism via cytochrome P450 (CYP) enzymes were determined to be 0.77 for CYP3A4/5 and 0.14 for CYP1A2 based on phenotyping studies in HLM. However, additional clinical studies are required to understand the potential contribution of CYP1A1. Approximately 83% of the dose was eliminated as N-methylpyrazolyl oxidative metabolites, with 52.1% of the dose excreted as M1 alone. Notably, M1 was not observed as a circulating metabolite in earlier metabolic profiling of human plasma from a multiple ascending study with unlabeled brepocitinib. Mechanistic studies revealed M1 was highly unstable in human plasma and phosphate buffer, undergoing chemical oxidation leading to loss of the 5-hydroxy-1-methylpyrazole moiety and formation of aminopyrimidine cleavage product M2. Time dependent inhibition and trapping studies with M1 yielded insights into the mechanism of this unusual and unexpected instability. Significance Statement This work describes the mass balance and metabolic profile of brepocitinib in human, a JAK1/TYK2 inhibitor being developed for treatment of autoimmune diseases.

Parametric optimisation of laser welding of stainless steel 316L

This paper presents numerical and experimental studies focused on the optimisation of laser welding parameters for AISI 316L stainless steel. The focus of the numerical studies was to obtain the mathematical model with the least time complexity and the highest fidelity. Based on the comparison of different mathematical models, a combination of two models, double ellipsoidal and conical models, was found to be optimal for the numerical simulation of the laser welding process. The studies were also complemented by material characterization studies for validation purpose. A pulse duration of 8 milliseconds and a current of 400 amperes with an average power of 380W were found to be the optimum parameters for laser welding of standard gauge 12 sheet of stainless steel AISI 316L. In addition, the effect of duty factor of the pulsed laser beam on the weld profile was also investigated and was found to be a major contributor to the optimisation process. The properties of the sample welded with the optimised set of parameters were also compared with the base metal, and based on the mechanical characterisation studies, it was found that the yield strength and hardness of the welded sample were improved, but the overall ductility was slightly reduced as compared to the base metal. The average weld zone size was also reduced by increasing the power density due to multiple reflections of the beam.

The effect of span length on the flexural properties of glass and basalt fiber reinforced sandwich structures with balsa wood core for sustainable shipbuilding

The current research aims to analyze the mechanical characterization of sandwich materials through a three-point flexural test. The sandwich structures in question composed of balsa wood as a core and four different types of fiber reinforced vinyl ester composite facesheets, namely Glass, Basalt, Glass/Carbon, and Basalt/Carbon. The sandwich panels were prepared using the vacuum infused processing method. The primary objective of this investigation is to explore the feasibility of utilizing basalt fibers for the production of structural parts in shipbuilding and replacing the already existed glass fibers. The flexural test was carried out with using three-point flexural test with varying the span length from 120 mm, 180 mm, 220 mm. Additionally, an analysis of variance (ANOVA) was then carried out to compare the mean values of properties deduced from these tests. The results showed that using basalt fibers instead of glass fiber reinforced enhanced the flexural stiffness of the sandwich structure. The flexural strength and modulus are shown to depend on span length and fiber type. The flexural modulus increases with an increase in span length. Similarly, flexural strength increases in glass fiber-based structures, while a slight reduction is observed in basalt fiber-reinforced structures the larger span length. The findings of this research suggest that basalt fibers hold potential as a replacement for glass fibers in producing structural components in shipbuilding. These results offer valuable information that can aid in the design and optimization of sandwich materials in shipbuilding.

Experimental and numerical mechanical characterisation of additively manufactured polymeric lattice structures under uniaxial tensile load

AbstractAdditive manufacturing enables the production of lighter, more robust components with intricate features like lattice structures. However, since the mechanical behaviour of lattice structures is not fully characterized, the application of such potential is limited today. The challenge with lattice structures tensile tests is defining a suitable design that fits the standard requirements and process characteristics. In the polymeric powder bed fusion process, the problem is to produce powder-free geometries and to avoid stress concentrations zones, adapting the specimen accordingly. In this regard, numerical simulation may provide insightful information and support the analysis of the deformation mechanisms. This paper analyses a new tensile sample for lattice structures using finite element analysis. The sample is designed following the EN ISO 527 standard prescriptions. An area with a controlled gradation of the lattice relative density is designed to ensure both powder-free voids and fracture localization within the lattice specimen gauge length. Experimental tests are performed to validate the numerical results using a modified body cubic centred topology with two different strut diameters. The specimens are produced in polyamide by powder bed AM process. Due to the complexity of the lattice design, a digital image correlation is used to compute the full range of strains at the macroscopic level. Experimental and numerical strain maps results showed a good agreement. The recorded deviation was attributed to the process-induced defect, such as the geometrical accuracy that, if compensated, boosted the capability of the numerical model to predict the mechanical behaviour of the lattice structure.

Delamination Initiation Identification of Mode II Based on AE Characterization of Composite Damage Mechanism

Delamination Initiation Identification of Mode II Based on AE Characterization of Composite Damage Mechanism

Structural analysis of small-scale 3D printed composite tidal turbine blades

The existing research literature lacks comprehensive investigations into assessing the structural performance of marine renewable energy conversion devices, particularly 3D printed turbine blades, which often rely solely on computational modelling without experimental validation methods and/or established mechanical characterization techniques. This leads to significant uncertainty regarding the performance of 3D printed turbine blades manufactured by additive manufacturing technology. This study aims to fill this gap by proposing a procedure for evaluating the structural integrity of commercial small-scale tidal turbine blade (5 KW) manufactured using fused filament fabrication 3D printing with a linear infill pattern. This is achieved by developing a combined experimental, hydrodynamic, and finite element approach with the view to inspect the micro-mechanical properties of representative volume elements of 3D printed microstructures using homogenization technique. The results of mechanical testing and hydrodynamic modelling are used to create a finite element model of the 3D printed blade, allowing for stress and failure analysis. Findings indicate that while integrating 3D printed materials into blade design via 3D printing technology is feasible, the choice of materials is limited to high stiffness composite filaments. Finally, experimental validation of numerical results, particularly full field strain distribution maps obtained by digital image correlation technique for flexural testing and laboratory-scale 3D printed blade, confirms the accuracy of the finite element results. Finite element-based homogenization techniques provide valuable insights into potential failure modes in 3D printed tidal turbine blades. However, the expedited calculation of orthotropic properties through finite element analysis proves to be a faster mechanical characterization method compared to experimental approaches. The proposed methodology in this study facilitates quicker iterative design of 3D printed blades, thereby reducing the need for repeated experiments and ultimately lowering manufacturing costs.

Mechanical and Physical Changes in Bio-Polybutylene-Succinate Induced by UVC Ray Photodegradation.

Bio-polybutylene succinate (PBS) is a biodegradable polymer obtained from renewable feedstock having physical-mechanical properties like traditional low-density polyethylene (LDPE). PBS is employed by many manufacturing sectors, from biomedical to agri-food and cosmetics. Although some studies have already evaluated the resistance of PBS to photodegradation caused by natural outdoor solar exposure (UVA-UVB), a systematic study on the resistance to degradation caused by exposure to UVC rays, which is the subject of this study, has not yet been carried out. PBS was exposed to UVC either neat or filled with 2% carbon black (CB). Mechanical and physical characterization (tensile, hardness, calorimetry, contact angle, morphology, and surface roughness analyses) indicates that the bulk and surface properties of the polymer matrix changes after exposure to UVC radiations, due to a severe degradation. However, the presence of carbon black compensates for the degradation phenomenon. Because UVC rays are used for the sterilization process, necessary in applications such as biomedical, cosmetic, pharmaceutical, food, and other products, a comparison of the protocol used in this paper with the literature's data has been reported and discussed.

Theoretical Modeling and Mechanical Characterization at Increasing Temperatures under Compressive Loads of Al Core and Honeycomb Sandwich

This work investigates the mechanical behavior under out-of-plane compression of the Al core and honeycomb sandwich at increasing temperatures of up to 300 °C. After the first introductive theoretical modeling on room-temperature compressive behavior, the experimental results at increasing temperatures up to 300 °C are presented and discussed. The analysis of the results shows that peak stress, plateau stress, and specific absorbed energy gradually decrease as the temperature increases. The final densification occurs always at the same strain level (around 75%). Sandwich honeycomb test temperatures have been limited to 200 °C for bonding problems of the skin to the sandwich due to the glue. The experimental and modeling results agree well at room temperature as well at increasing temperatures. The results can provide useful information to choose base materials for greater energy absorption at increasing temperatures.

Seismic performance assessment of the church of SS. Annunziata in Paestum through finite element analysis

This paper presents a comprehensive study on the Church of Ss. Annunziata in Paestum (Italy), focusing on its historical reconstruction, current condition analysis, and dynamic characterization. An extensive photogrammetric survey employing terrestrial and aerial photography was conducted to establish an accurate geometric model of the church. Subsequently, the model was set up into Abaqus ambiance for dynamic characterization, material modelling and assessment of potential local failure mechanisms through nonlinear dynamic analysis under varying seismic intensities. The research provides valuable insights into the structural behaviour of the church and its vulnerability to seismic events, aiding in informed decision-making for preservation and restoration efforts.